Bone Marrow and Flow Cytometry

Specimen Type Methodology Requirements Containers Collection Protocols
Peripheral Blood Flow Immunophenotype 2-5 ml of blood, 1 freshly prepared smear (preferred) Green Top (Sodium Heparin), Purple Top (EDTA), Yellow Top (ACD) Label the tube with the patient’s name, the date and time that the specimen was drawn. Keep the sample at room temperature and use Flow Cytometry¬†shipping package for transport.
Bone Marrow Aspirate Flow Immunophenotype 1-3 ml of bone marrow, 1 freshly prepared smear (preferred) Green Top (Sodium Heparin) (Preferred), or Purple Top (EDTA), or Yellow Top (ACD) Label the tube with the patient’s name, the date and time that the specimen was drawn.¬† Do not draw more than 2 mls /tube.Keep the sample at room temperature and use Flow Cytometry shipping package for transport.
Bone Marrow Biopsy Core Flow Immunophenotype 1-2 cm bone core RPMI 1640 or other tissue culture media, Sterile saline solution Immerse the biopsy into the RPMI 1640. Keep the sample at room temperature refrigerated and use Flow Cytometry shipping package for transport.
Fresh Tissue Biopsy Flow Immunophenotype Approximately 0.5 cmx0.5cmx0.5cm, H&E slide, if available Tissue culture media (RPMI 1640/ Hank’s solution), Sterile saline solution Cut tissue specimen in thin slices (no more than 1.0 mm thick). Immerse the tissue into the RPMI 1640. Keep the sample at room temperature or refrigerated and use Flow Cytometry shipping package for transport.
Fine Needle Aspirate Flow Immunophenotype As many needle passes as possible Sterile tissue culture media (RPMI 1640) Irrigate the collected aspirate into RPMI 1640. Keep the sample at room temperature or refrigerated and use Flow Cytometry shipping package for transport.
Body Fluids Flow Immunophenotype Provide approx. 50 ml or as much as possible (CSF at least 2.0 ml) Sterile container Keep the sample at room temperature or refrigerated and use Flow Cytometry shipping package for transport.